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Science 11 December 1981:
Vol. 214. no. 4526, pp. 1244 - 1246
DOI: 10.1126/science.6272397
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Articles

Science, Vol 214, Issue 4526, 1244-1246
Copyright © 1981 by American Association for the Advancement of Science

articles

Integration and stable germ line transmission of genes injected into mouse pronuclei

JW Gordon and FH Ruddle

Genetic material has been successfully transferred into the genomes of newborn mice by injection of that material into pronuclei of fertilized eggs. Initial results indicated two patterns of processing the injected DNA: one in which the material was not integrated into the host genome, and another in which the injected genes became associated with high molecular weight DNA. These patterns are maintained through further development to adulthood. The evidence presented indicates the covalent association of injected DNA with host sequences, and transmission of such linked sequences in a Mendelian distribution to two succeeding generations of progeny.


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