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Science 4 December 1981:
Vol. 214. no. 4525, pp. 1125 - 1129
DOI: 10.1126/science.6272395
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Articles

Science, Vol 214, Issue 4525, 1125-1129
Copyright © 1981 by American Association for the Advancement of Science

articles

Cloned viral protein vaccine for foot-and-mouth disease: responses in cattle and swine

DG Kleid, D Yansura, B Small, D Dowbenko, DM Moore, MJ Grubman, PD McKercher, DO Morgan, BH Robertson, and HL Bachrach

A DNA sequence coding for the immunogenic capsid protein VP3 of foot-and-mouth disease virus A12, prepared from the virion RNA, was ligated to a plasmid designed to express a chimeric protein from the Escherichia coli tryptophan promoter-operator system. When Escherichia coli transformed with this plasmid was grown in tryptophan-depleted media, approximately 17 percent of the total cellular protein was found to be an insoluble and stable chimeric protein. The purified chimeric protein competed equally on a molar basis with VP3 for specific antibodies to foot-and-mouth disease virus. When inoculated into six cattle and two swine, this protein elicited high levels of neutralizing antibody and protection against challenge with foot-and-mouth disease virus.


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