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Science 20 November 1981:
Vol. 214. no. 4523, pp. 921 - 923
DOI: 10.1126/science.6272392
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Articles

Science, Vol 214, Issue 4523, 921-923
Copyright © 1981 by American Association for the Advancement of Science

articles

Renin and angiotensin: the complete system within the neuroblastoma x glioma cell

MC Fishman, EA Zimmerman, and EE Slater

Cells of the homogeneous hybrid line neuroblastoma x glioma (NG108-15) have many neuronal properties. Immunocytochemical tests show that they contain both immunoreactive renin and angiotensin; direct radioimmunoassays show that they are positive for renin, angiotensin I, and angiotensin II; enzymatic assays show that they contain angiotensinogen and converting enzyme as well. The renin appears to be present in an enzymatically inactive form that can be activated by trypsin and then blocked by antiserum to purified mouse submaxillary renin. Renin concentration and activity are increased by enhancing cellular differentiation with dibutyryl cyclic adenosine monophosphate or by serum withdrawal. These findings demonstrate a complete renin-angiotensin system within these neuron-like cells, and suggest that activation of intracellular renin could generate angiotensin II.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Cellular Biology of the Renin-Angiotensin Systems.
R. N. Re (1984)
Arch Intern Med 144, 2037-2041
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