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Science 20 November 1981:
Vol. 214. no. 4523, pp. 916 - 919
DOI: 10.1126/science.6272391
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Articles

Science, Vol 214, Issue 4523, 916-919
Copyright © 1981 by American Association for the Advancement of Science

articles

Cloned poliovirus complementary DNA is infectious in mammalian cells

VR Racaniello and D Baltimore

A complete, cloned complementary DNA copy of the RNA genome of poliovirus was constructed in the Pst I site of the bacterial plasmid pBR322. Cultured mammalian cells transfected with this hybrid plasmid produced infectious poliovirus. Cells transfected with a plasmid which lacked the first 115 bases of the poliovirus genome did not produce virus.


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