Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 23 October 1981:
Vol. 214. no. 4519, pp. 458 - 460
DOI: 10.1126/science.7291986
Prev | Table of Contents | Next

Articles

Science, Vol 214, Issue 4519, 458-460
Copyright © 1981 by American Association for the Advancement of Science

articles

Voltage clamp studies in macrophages from mouse spleen cultures

EK Gallin

Voltage clamp studies of macrophages from cultures of mouse spleen macrophages produced N-shaped steady-state current-voltage curves containing a region of negative slope resistance. Some macrophages exhibit two stable states of membrane potential, having current-voltage relationships that cross the voltage axis at three points. Outward currents that turn on at voltages of +15 millivolts or greater were noted in several cells. The addition of barium chloride to the bathing medium abolished the negative slope resistance and reduced the inward currents in response to hyperpolarizing voltage steps. These data provide direct evidence that macrophages exhibit at least tow different voltage-dependent conductances and demonstrate that voltage clamp techniques can be useful in studying the membrane properties of leukocytes.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Voltage-Gated Proton Channels and Other Proton Transfer Pathways.
T. E. Decoursey (2003)
Physiol Rev 83, 475-579
   Abstract »    Full Text »    PDF »
Action potentials in macrophages derived from human monocytes.
F. McCann, J. Cole, P. Guyre, and J. Russell (1983)
Science 219, 991-993
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)