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Science 1 May 1981:
Vol. 212. no. 4494, pp. 549 - 551
DOI: 10.1126/science.7209549
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Articles

Science, Vol 212, Issue 4494, 549-551
Copyright © 1981 by American Association for the Advancement of Science

articles

Tritiated thymidine incorporation does not measure DNA synthesis in ribavirin-treated human cells

JC Drach, MA Thomas, JW Barnett, SH Smith, and C Shipman Jr

When the incorporation of tritiated thymidine into acid insoluble material was measured, ribavirin appeared to be a potent inhibitor of DNA synthesis in KB cells and human lymphocytes. Inhibition was nearly 100-fold less, however, when DNA synthesis was measured by incorporation of phosphorus-32-labeled phosphate or by DNA fluorescence. The potent inhibition detected by incorporation of tritiated thymidine into DNA actually was the result of a potent effect on the labeling of deoxythymidine triphosphate, not on the synthesis of DNA.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Ribavirin suppresses eIF4E-mediated oncogenic transformation by physical mimicry of the 7-methyl guanosine mRNA cap.
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The Role of Mast Cell Tryptase in Regulating Endothelial Cell Proliferation, Cytokine Release, and Adhesion Molecule Expression: Tryptase Induces Expression of mRNA for IL-1{beta} and IL-8 and Stimulates the Selective Release of IL-8 from Human Umbilical Vein Endothelial Cells.
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Ribavirin antagonizes the effect of azidothymidine on HIV replication.
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Science 235, 1376-1379
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