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Science 5 December 1980:
Vol. 210. no. 4474, pp. 1131 - 1133
DOI: 10.1126/science.7444440
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Articles

Science, Vol 210, Issue 4474, 1131-1133
Copyright © 1980 by American Association for the Advancement of Science

articles

Relation of mammalian sperm chromatin heterogeneity to fertility

DP Evenson, Z Darzynkiewicz, and MR Melamed

Flow cytometry of heated sperm nuclei revealed a significant decrease in resistance to in situ denaturation of spermatozoal DNA in samples from bulls, mice, and humans of low or questionable fertility when compared with others of high fertility. Since thermal denaturation of DNA in situ depends on chromatin structure, it is assumed that changes in sperm chromatin conformation may be related to the diminished fertility. Flow cytometry of heated sperm nuclei may provide a new and independent determinant of male fertility.


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