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Science 19 September 1980:
Vol. 209. no. 4463, pp. 1428 - 1430
DOI: 10.1126/science.6158095
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Articles

Science, Vol 209, Issue 4463, 1428-1430
Copyright © 1980 by American Association for the Advancement of Science

articles

A technique for expressing eukaryotic genes in bacteria

L Guarente, TM Roberts, and M Ptashne

Methods are described that allow efficient expression in Escherichia coli of cloned eukaryotic genes. The methods require that the coding sequence of the gene in question be available in a form uninterrupted by intervening sequences (for example, as a complementary DNA clone). The gene products are synthesized unfused to other amino acid sequences. The genetic manipulations are simple, and require the plasmids described and commercially available enzymes.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Rabies virus glycoprotein analogs: biosynthesis in Escherichia coli.
E Yelverton, S Norton, J. Obijeski, and D. Goeddel (1983)
Science 219, 614-620
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