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Science 19 September 1980:
Vol. 209. no. 4463, pp. 1414 - 1422
DOI: 10.1126/science.7414320
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Articles

Science, Vol 209, Issue 4463, 1414-1422
Copyright © 1980 by American Association for the Advancement of Science

articles

Altering genotype and phenotype by DNA-mediated gene transfer

A Pellicer, D Robins, B Wold, R Sweet, J Jackson, I Lowy, JM Roberts, GK Sim, S Silverstein, and R Axel

Transformation, or DNA-mediated gene transfer, permits the introduction of new genetic information into a cell and frequently results in a change in phenotype. The transforming DNA is ultimately integrated into a recipient cell chromosome. No unique chromosomal locations are apparent, different lines contain the transforming DNA on different chromosomes. Expression of transformed genes frequently results in the synthesis of new polypeptide products which restore appropriate mutant cells to the wild-type phenotype. Thus transformation provides an in vivo assay for the functional role of DNA sequence organization about specific genes. Transforming genes coding for selectable functions, such as adenine phosphoribosyltransferase or thymidine kinase, have now been isolated by utilizing transformation in concert with molecular cloning. Finally, transformation may provide a general approach to the analysis of complex heritable phenotypes by permitting the distinction between phenotypic changes without concomitant changes in DNA and functional genetic rearrangements.


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