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Science 19 September 1980:
Vol. 209. no. 4463, pp. 1353 - 1360
DOI: 10.1126/science.6968091
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Articles

Science, Vol 209, Issue 4463, 1353-1360
Copyright © 1980 by American Association for the Advancement of Science

articles

Mouse immunoglobulin D: messenger RNA and genomic DNA sequences

PW Tucker, CP Liu, JF Mushinski, and FR Blattner

The molecular structure of a mouse immunoglobulin D from a plasmacytoma tumor and that of the normal mouse gene coding for immunoglobulin D are presented. The DNA sequence results indicate an unusual structure for the tumor delta chain in two respects: (i) Only two constant (C) region domains, termed C delta 1 and C delta 3 by homology considerations, are found; the two domains are separated by an unusual hinge region C delta H that lacks cysteine residues and thus cannot provide the covalent cross-links between heavy chains typically seen in immunoglobulins. The two domains and hinge are all coded on separate exons. (ii) At the carboxyl end of the delta chain there is a stretch of 26 amino acids that is coded from an exon located 2750 to 4600 base pairs downstream from the rest of the gene. Analogy with immunoglobulin M suggests that this distally coded segment C delta DC may have a membrane-binding function; however, it is only moderately hydrophobic. A fifth potential exon (C delta AC), located adjacent to the 3' (carboxyl) end of C delta 3, could code for a stretch of 49 amino acids. The tumor's expression of the delta gene may be aberrant, but the simplest interpretation would be that this tumor expresses one of the several biologically significant forms of the delta chain.


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Differential Segmental Flexibility and Reach Dictate the Antigen Binding Mode of Chimeric IgD and IgM: Implications for the Function of the B Cell Receptor.
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Physical Mapping of the Bovine Immunoglobulin Heavy Chain Constant Region Gene Locus.
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A novel chimeric Ig heavy chain from a teleost fish shares similarities to IgD.
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Human immunoglobulin D: genomic sequence of the delta heavy chain.
M. White, A. Shen, C. Word, P. Tucker, and F. Blattner (1985)
Science 228, 733-737
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Mapping of heavy chain genes for mouse immunoglobulins M and D.
C. Liu, P. Tucker, J. Mushinski, and F. Blattner (1980)
Science 209, 1348-1353
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