Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 25 July 1980:
Vol. 209. no. 4455, pp. 510 - 513
DOI: 10.1126/science.7394518
Prev | Table of Contents | Next

Articles

Science, Vol 209, Issue 4455, 510-513
Copyright © 1980 by American Association for the Advancement of Science

articles

Calcium regulation during stimulus-secretion coupling: continuous measurement of intracellular calcium activities

J O'Doherty, SJ Youmans, WM Armstrong, and RJ Stark

Accurate measurements of intracellular calcium activities in salivary gland epithelial cells of the insect Phormia regina were obtained with microelectrodes in which N,N'-di(11-ethoxycarbonyl)undecyl-N,N'-4,5-tetramethyl-3,6-dioxaoctane diacid diamide wsa incorporated in a liquid membrane system. When calibrated in solutions approximating the ionic concentration of the cell interior, these microelectrodes gave rapid stable responses that were linear functions of the logarithm of calcium activities and were not affected by potassium, sodium and magnesium. Continuous monitoring of calcium activities during serotonin-induced saliva release provided direct evidence of hormonal influence on transmembrane calcium movement and spontaneous regulation of intracellular calcium by stimulated cells.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Cytoplasmic calcium in the mediation of macula densa tubulo-glomerular feedback responses.
P. Bell and L. Navar (1982)
Science 215, 670-673
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)