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Science 18 July 1980:
Vol. 209. no. 4454, pp. 391 - 394
DOI: 10.1126/science.6992276
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Articles

Science, Vol 209, Issue 4454, 391-394
Copyright © 1980 by American Association for the Advancement of Science

articles

Survival of Escherichia coli host-vector systems in the mammalian intestine

SB Levy, B Marshall, D Rowse-Eagle, and A Onderdonk

Survival in the mouse and human intestine of Escherichia coli host-vector systems used and proposed for recombinant DNA technology was assessed. There was no detectable survival of severely disabled E. coli K12 strain X1776 in mice or in human subjects 24 hours after ingestion. The same strain bearing the plasmid pBR322, however, was recovered from human subjects for 4 days in amounts of six organisms for every million ingested. Nondisabled E. coli K12 strain X1666, with or without pBR322, survived in 10(4)-fold greater numbers and for 2 days longer, with better recovery of the plasmid-containing derivative. Although the plasmid-bearing strains were recovered for longer periods, no intestinal colonization was noted. Despite the presence of pBR322 for a maximum of 6 days in the human intestine, there was no evidence that it was transferred from either bacterial host to endogenous aerobic fecal bacteria.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Examination of Salmonella and Escherichia coli Translocation from Hog Manure to Forage, Soil, and Cattle Grazed on the Hog Manure-treated Pasture.
R. Holley, J. Walkty, G. Blank, M. Tenuta, K. Ominski, D. Krause, and L.-K. Ng (2008)
J. Environ. Qual. 37, 2083-2092
   Abstract »    Full Text »    PDF »
Human Growth Hormone and Other Proteins Prepared by Recombinant DNA Technology.
S. L. BLETHEN and V. V. WELDON (1982)
Arch Pediatr Adolesc Med 136, 669-671
   Abstract »    PDF »


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