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Science 7 December 1979:
Vol. 206. no. 4423, pp. 1192 - 1194
DOI: 10.1126/science.505006
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Articles

Science, Vol 206, Issue 4423, 1192-1194
Copyright © 1979 by American Association for the Advancement of Science

articles

Different nucleosome structures on transcribing and nontranscribing ribosomal gene sequences

EM Johnson, GR Campbell, and VG Allfrey

Monomeric DNA lengths from Physarum nuclear chromatin occur in two subunit forms which differ from each other and from higher oligomers of nucleosomes in content of transcribed ribosomal DNA sequences. Labeled DNA restriction fragments from ribosomal RNA coding regions reanneal most rapidly with DNA from a monomeric subunit fraction. A particles, isolated from growing plasmodia and containing 144 base pairs of DNA in an extended conformation. Higher oligomers of nucleosomes are depleted in sequences from transcribing gene regions but are enriched in sequences from the nontranscribed central spacer of the ribosomal DNA palindrome. Nucleosome configuration on two 26S gene intervening sequences resembles that on adjacent coding regions.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Assembly into chromatin and subtype-specific transcriptional effects of exogenous linker histones directly introduced into a living Physarum cell.
C Thiriet and J. Hayes (2001)
J. Cell Sci. 114, 965-973
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