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Science 30 November 1979:
Vol. 206. no. 4422, pp. 1081 - 1083
DOI: 10.1126/science.227059
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Articles

Science, Vol 206, Issue 4422, 1081-1083
Copyright © 1979 by American Association for the Advancement of Science

articles

A sign inversion mechanism for enzymatic supercoiling of DNA

PO Brown and NR Cozzarelli

Both the introduction and the removal of supertwists by DNA gyrase change the linking number of DNA in steps of two. This surprising finding provides strong evidence that gyrase acts by a mechanism, called sign inversion, whereby a positive supercoil is directly inverted into a negative one via a transient double-strand break.


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Escherichia coli Type-1 Topoisomerases: Identification, Mechanism, and Role in Recombination.
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In Vitro Study of Illegitimate Recombination: Involvement of DNA Gyrase.
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DNA gyrase and the supercoiling of DNA.
N. Cozzarelli (1980)
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