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Science 2 November 1979:
Vol. 206. no. 4418, p. 585
DOI: 10.1126/science.206.4418.585
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Articles

Maternal Haploids of Nicotiana tabacum L. from Seed

L. G. BURK 1, D. U. GERSTEL 2, and E. A. WERNSMAN 2

1 Tobacco Research Laboratory, Agricultural Research, Science and Education Administration, U.S. Department of Agriculture, Oxford, North Carolina 27565
2 Department of Crop Science, North Carolina State University, Raleigh 27650

Abundant seeds of high germinability are obtained when Nicotiana tabacum is pollinated by Nicotiana africana. Most of the seedlings die at the cotyledonary stage. The remaining seedlings are viable F1 hybrids or maternal haploids that can be easily distinguished. This simple method of producing Nicotiana tabacum haploids offers an alternative to anther culture.

Submitted on April 5, 1979
Revised on May 24, 1979


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