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Science 15 June 1979:
Vol. 204. no. 4398, pp. 1226 - 1228
DOI: 10.1126/science.36662
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Articles

Science, Vol 204, Issue 4398, 1226-1228
Copyright © 1979 by American Association for the Advancement of Science

articles

Separation of D and L amino acids by liquid chromatography: use of chiral eluants

PE Hare and E Gil-Av

An aqueous eluant containing a chiral copper-proline complex effects the separation of underivatized amino acid enantiomers on an ion-exchange column. The stereoselectivity is ascribed to differences in stability of the diastereomeric amino acid-copper compexes formed in solution. A simple change in the chirality of the eluant reverses the order of the enantiomer elution. For detection and quantification of picomole amounts of amino acids, the eluant is monitored for fluorescence after reaction with o-phthalaldehyde, a reagent insensitive to proline but highly sensitive for amino acids containing a primary amino group.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Electrokinetic Separation of Chiral Compounds.
E. GASSMANN, J. E. KUO, and R. N. ZARE (1985)
Science 230, 813-814
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