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Science 4 May 1979:
Vol. 204. no. 4392, pp. 503 - 506
DOI: 10.1126/science.432654
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Articles

Science, Vol 204, Issue 4392, 503-506
Copyright © 1979 by American Association for the Advancement of Science

articles

Chicken gizzard: relation between calcium-activated phosphorylation and contraction

PE Hoar, WG Kerrick, and PS Cassidy

Of the proteins in mechanically disrupted chicken gizzard fibers (no functional sarcolemma) only the 20,000-dalton light chains of myosin underwent large Ca2+-and Sr2+-dependent changes in phosphorylation. Phosphorylation closely corresponded with the Ca2+- and Sr2+-activated tensions. Adenosine 5'-O (3'-thiotriphosphate) only in the presence of Ca2+ induced irreversible Ca2+-insensitive activation of tension and thiophosphorylation of the 20,000-dalton light chains, and blocked incorporation of 32P from [gamma-32P]adenosine triphosphate into the myosin light chains.


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