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Science 6 April 1979:
Vol. 204. no. 4388, pp. 75 - 77
DOI: 10.1126/science.204.4388.75
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Articles

Crystalline Ribulose 1,5-Bisphosphate Carboxylase-Oxygenase from Spinach

SARJIT JOHAL 1 and DON P. BOURQUE 2

1 Department of Nutrition and Food Science, University of Arizona, Tucson 85721
2 Department of Biochemistry, University of Arizona

Spinach fraction I protein (ribulose 1,5-bisphosphate carboxylase-oxy genase, E.C. 4.1.1.39) was crystallized on both an analytical and a preparative scale by vapor diffusion with polyethylene glycol (molecular weight, 6000) used as the precipitant. The identity of the crystalline material with fraction I protein was shown by gel electrophoresis in the presence of sodium dodecyl sulfate and immunological properties. The carboxylase and oxygenase activities copurify during crystallization, and the crystalline enzyme lacks copper and iron.

Submitted on August 11, 1978
Revised on October 24, 1978

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Science. ISSN 0036-8075 (print), 1095-9203 (online)