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Science 2 March 1979:
Vol. 203. no. 4383, pp. 887 - 892
DOI: 10.1126/science.217088
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Articles

Science, Vol 203, Issue 4383, 887-892
Copyright © 1979 by American Association for the Advancement of Science

articles

Molecular cloning of polyoma virus DNA in Escherichia coli: lambda phage vector system

HW Chan, MA Israel, CF Garon, WP Rowe, and MA Martin

The biological activity of recombinant phage and recombinant phage DNA containing monomeric or dimeric polyoma DNA inserts was examined in mice and cultured mouse cells. Recombinant preparations containing a single copy of viral DNA were invariably noninfectious; molecules containing a dimeric polyoma DNA insert were at least seven orders of magnitude less infectious than polyoma virions after parenteral inoculation. No infection was detected with any recombinant preparation after oral administration.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Isolation of mutants of an animal virus in bacteria.
K. Peden, J. Pipas, S Pearson-White, and D Nathans (1980)
Science 209, 1392-1396
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Deletion Mutants of SV40 That Affect the Structure of Viral Tumor Antigens.
J. M. Pipas, S. P. Adler, K. W. C. Peden, and D. Nathans (1980)
Cold Spring Harb Symp Quant Biol 44, 285-291
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Molecular cloning of polyoma virus DNA in Escherichia coli: oncogenicity testing in hamsters.
M. Israel, H. Chan, M. Martin, and W. Rowe (1979)
Science 205, 1140-1142
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Molecular cloning of polyoma virus DNA in Escherichia coli: plasmid vector system.
M. Israel, H. Chan, W. Rowe, and M. Martin (1979)
Science 203, 883-887
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