Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 22 December 1978:
Vol. 202. no. 4374, pp. 1284 - 1289
DOI: 10.1126/science.725604
Prev | Table of Contents | Next

Articles

Science, Vol 202, Issue 4374, 1284-1289
Copyright © 1978 by American Association for the Advancement of Science

articles

Cloning human fetal gamma globin and mouse alpha-type globin DNA: characterization and partial sequencing

O Smithies, AE Blechl, K Denniston-Thompson, N Newell, JE Richards, JL Slightom, PW Tucker, and FR Blattner

Two globin-related clones isolated from collections of bacteriophages containing unfractionated Eco RI fragments of human and mouse DNA were characterized. Charon3AHs51.1Hbgamma includes 2.7 kilobase pairs of human DNA containing a large part of a fetal gamma globin chain structural gene; Charon 3AMm30.5 includes 4.7 kilobase pairs of mouse DNA related to alpha globin. The human fetal gamma globin gene has within its coding region two intervening sequences of noncoding DNA, IVS 1 and IVS 2, of approximately 1-0 and 900 base pairs. Sequence IVS 1 is located at the position of one of the two intervening sequences occurring in adult globin genes; IVS 2 is located at the position of the other.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Structure and in vitro transcription of human globin genes.
N. Proudfoot, M. Shander, J. Manley, M. Gefter, and T Maniatis (1980)
Science 209, 1329-1336
   Abstract »    PDF »
J genes for heavy chain immunoglobulins of mouse.
N Newell, J. Richards, P. Tucker, and F. Blattner (1980)
Science 209, 1128-1132
   Abstract »    PDF »
Disorders of human hemoglobin.
A Bank, J. Mears, and F Ramirez (1980)
Science 207, 486-493
   Abstract »    PDF »
Sequence of the cloned gene for the constant region of murine gamma 2b immunoglobulin heavy chain.
P. Tucker, K. Marcu, N Newell, J Richards, and F. Blattner (1979)
Science 206, 1303-1306
   Abstract »    PDF »
Comparison of total sequence of a cloned rabbit beta-globin gene and its flanking regions with a homologous mouse sequence.
A van Ooyen, J van den Berg, N Mantei, and C Weissmann (1979)
Science 206, 337-344
   Abstract »    PDF »
Molecular Genetics of Human Hemoglobin Synthesis.
B. G. FORGET (1979)
Ann Intern Med 91, 605-616
   Abstract »    PDF »
Cloning human fetal gamma globin and mouse alpha-type globin DNA: preparation and screening of shotgun collections.
F. Blattner, A. Blechl, K Denniston-Thompson, H. Faber, J. Richards, J. Slightom, P. Tucker, and O Smithies (1978)
Science 202, 1279-1284
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)