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Science 17 November 1978:
Vol. 202. no. 4369, pp. 763 - 765
DOI: 10.1126/science.715441
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Articles

Science, Vol 202, Issue 4369, 763-765
Copyright © 1978 by American Association for the Advancement of Science

articles

Growth of infective forms of Trypanosoma rhodesiense in vitro, the causative agent of African trypanosomiasis

GC Hill, SP Shimer, B Caughey, and LS Sauer

A new approach to the culture of African trypanosomes led to the growth of the infective forms of the causative agent of human African trypanosomiasis. Infective cultures of Trypanosoma rhodesiense were initiated and maintained in vitro on Chinese hamster lung cells. By changing daily one-third of the Hepes-buffered RPMI 1640 medium containing 20 percent fetal bovine serum, the trypanosome numbers increased to 3 X 10(6) to 5 X 10(6) cells per milliliter. After 80 days in vitro at 37 degrees C, the cultured trypomastigotes are infective for mice and rats and morphologically similar to bloodstream trypomastigotes in having a subterminal kinetoplast and a surface coat. In addition, they possess L-alpha-glycerophosphate oxidase, the predominant steady-state terminal oxidase of bloodstream trypomastigotes.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Cultivation of Clinically Significant Hemoflagellates.
F. L. Schuster and J. J. Sullivan (2002)
Clin. Microbiol. Rev. 15, 374-389
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Primary murine bone marrow cultures support continuous growth of infectious human trypanosomes.
A. Balber (1983)
Science 220, 421-423
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