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Science 18 August 1978:
Vol. 201. no. 4356, pp. 651 - 652
DOI: 10.1126/science.675253
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Articles

Science, Vol 201, Issue 4356, 651-652
Copyright © 1978 by American Association for the Advancement of Science

articles

Stimulated DNA synthesis in frog nuclei by cytoplasmic extracts of temperature-sensitive mammalian cells

J Floros, H Chang, and R Baserga

Cytoplasmic extracts of proliferating cells stimulate DNA synthesis in isolated nuclei of Xenopus laevis liver. When tested by the same assay, cytoplasmic extracts of resting cells are completely inactive. When cytoplasmic extracts are prepared from cell cycle-specific temperature-sensitive mutants arrestd in the G1 phase of the cell cycle by the nonpermissive temperature, they also fail to stimulate DNA synthesis in frog nuclei. The results indicate that, to stimulate DNA synthesis in isolated frog nuclei, essentially all information of G1 cells must be present.




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Science. ISSN 0036-8075 (print), 1095-9203 (online)