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Science 11 August 1978:
Vol. 201. no. 4355, pp. 529 - 531
DOI: 10.1126/science.208156
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Articles

Science, Vol 201, Issue 4355, 529-531
Copyright © 1978 by American Association for the Advancement of Science

articles

Virus-induced diabetes mellitus: reovirus infection of pancreatic beta cells in mice

T Onodera, AB Jenson, JW Yoon, and AL Notkins

Reovirus type 3, passaged in pancreatic beta-cell cultures, produced an insulitis when inoculated into 1- to 2-week-old mice. By means of a double-label antibody technique, in which we used fluorescein-labeled antibody to reovirus and rhodamine-labeled antibody to insulin, reovirus antigens were found in beta cells. By electron microscopy, viral particles in different stages of morphogenesis were observed in insulin-containing beta cells but not glucagon-containing alpha cells. The infection resulted in destruction of beta cells, reduction in the insulin content of the pancreas, and alteration in the host's capacity to respond normally to a glucose tolerance test.


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