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ArticlesCopyright © 1978 by American Association for the Advancement of Science
Reversed-phase, high-pressure liquid chromatography of peptides and proteins with ion-pairing reagents
Reversed-phase, high-pressure liquid chromatography has been successfully applied to the analysis of peptides and proteins by the addition of hydrophilic (for example, phosphoric acid) or hydrophobic (for example, hexanesulfonic acid) ion-pairing reagents, or both, to the mobile phase. Examples described included proteins such as insulin, glucagon, and 1-24 ACTH pentaacetate (ACTH is adrenocorticotrophic hormone).
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Science. ISSN 0036-8075 (print), 1095-9203 (online)