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Science 9 June 1978:
Vol. 200. no. 4346, pp. 1168 - 1170
DOI: 10.1126/science.206966
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Articles

Science, Vol 200, Issue 4346, 1168-1170
Copyright © 1978 by American Association for the Advancement of Science

articles

Reversed-phase, high-pressure liquid chromatography of peptides and proteins with ion-pairing reagents

WS Hancock, CA Bishop, RL Prestidge, DR Harding, and MT Hearn

Reversed-phase, high-pressure liquid chromatography has been successfully applied to the analysis of peptides and proteins by the addition of hydrophilic (for example, phosphoric acid) or hydrophobic (for example, hexanesulfonic acid) ion-pairing reagents, or both, to the mobile phase. Examples described included proteins such as insulin, glucagon, and 1-24 ACTH pentaacetate (ACTH is adrenocorticotrophic hormone).


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Identification and Quantification of Glycoproteins Using Ion-Pairing Normal-phase Liquid Chromatography and Mass Spectrometry.
W. Ding, H. Nothaft, C. M. Szymanski, and J. Kelly (2009)
Mol. Cell. Proteomics 8, 2170-2185
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Physalaemin: an amphibian tachykinin in human lung small-cell carcinoma.
L. Lazarus, R. DiAugustine, G. Jahnke, and O Hernandez (1983)
Science 219, 79-81
   Abstract »    PDF »


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