Separation of the Sperm Agglutinin and the Acrosome Reaction-Inducing Substance in Egg Jelly of Starfish
YOHKO UNO 1 and
MOTONORI HOSHI 1
1 Biochemical Laboratory, Institute of Low Temperature Science, Hokkaido University, Sapporo 060, Japan
The egg jelly of the starfish Asterias amurensis was separated into the fractions J1, J2, and J3 on a Sephadex G-100 column. The Jl fraction induced the acrosome reaction and J2 induced sperm agglutination. Chemical analysis and chromatography revealed that sperm agglutinin is similar to asterosaponin A.
Submitted on September 20, 1977