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Science 24 February 1978:
Vol. 199. no. 4331, pp. 902 - 904
DOI: 10.1126/science.146257
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Articles

Science, Vol 199, Issue 4331, 902-904
Copyright © 1978 by American Association for the Advancement of Science

articles

Membrane enzymes: artifacts in Arrhenius plots due to temperature dependence of substrate-binding affinity

Silvius JR, BD Read, and RN McElhaney

For the membrane sodium-stimulated magnesium-adenosinetriphosphatase of Acholeplasma laidlawii B both the Vmax and Km values in the Michaelis equation very strongly with temperature. Simulations of Arrhenius plots show that an enzyme with a temperature-dependent Km can yield a variety of Arrhenius plot artifacts, most notably erroneous "breaks," if activity is assayed at a fixed substrate concentration.




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Science. ISSN 0036-8075 (print), 1095-9203 (online)