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Science 27 January 1978:
Vol. 199. no. 4327, pp. 425 - 426
DOI: 10.1126/science.619462
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Articles

Science, Vol 199, Issue 4327, 425-426
Copyright © 1978 by American Association for the Advancement of Science

articles

Far-ultraviolet stopped-flow circular dichroism

J Luchins and S Beychok

A stopped-flow circular dichroism instrument, with a total accessible wavelength range of 200 to 750 nanometers, has been constructed and provides a spectroscopic method for kinetic investigations of a wide array of fast reactions in which optical activity changes in absorbing regions are involved. An important biochemical application depends on the far-ultraviolet capability, which allows observation of the rapid alterations in backbone conformation associated with folding and unfolding reactions of proteins. Results obtained by following two such reactions at 222 nanometers represent direct monitoring by circular dichroism of rapid secondary structure changes in proteins.




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Science. ISSN 0036-8075 (print), 1095-9203 (online)