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Science 9 December 1977:
Vol. 198. no. 4321, pp. 1038 - 1040
DOI: 10.1126/science.337490
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Articles

Science, Vol 198, Issue 4321, 1038-1040
Copyright © 1977 by American Association for the Advancement of Science

articles

Immunofluorescence localization of proteins of high molecular weight along intracellular microtubules

P Sherline and K Schiavone

To help clarify the role, in cytoplasmic microtubule function, of the proteins of high molecular weight that coassemble with tubulin in vitro, a monospecific antibody against the proteins of high molecular weight was prepared from the serum of immunized rabbits by affinity chromatography. With indirect immunofluorescence we found that the protein in both cultured neuroblastoma cells and 3T3 cells in distributed in an extensive filamentous array that originates at sites near the nucleus and extends to the cell periphery or, in neuroblastoma cells, gathers into bundles which enter neurites. No filaments were seen with nonspecific antibodies from serum taken before immunization, and prior incubation of the specific antibody with purified protein of high molecular weight (but not tubulin) prevented filament visualization. The filamentous pattern in 3T3 cells was disrupted by colchicine. This evidence indicates that the proteins of high molecular weight are found in cells in association with cytoplasmic microtubules.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Immunoreactive Forms of Human Erythrocyte Ankyrin Are Localized in Mitotic Structures in Cultured Cells and Are Associated with Microtubules in Brain.
V. Bennett and J. Davis (1982)
Cold Spring Harb Symp Quant Biol 46, 647-657
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