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Science 12 August 1977:
Vol. 197. no. 4304, pp. 671 - 674
DOI: 10.1126/science.877583
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Articles

Science, Vol 197, Issue 4304, 671-674
Copyright © 1977 by American Association for the Advancement of Science

articles

DNA strand scission by benzo[a]pyrene diol epoxides

HB Gamper, AS Tung, K Straub, JC Bartholomew, and M Calvin

Syn-and anti-benzo[a]pyrene diol epoxides elicit a concentration-dependent nicking of superhelical Col E1 DNA in an in vitro reaction monitored by agarose gel electrophoresis and electron microscopy. This strand scission represents less than 1 percent of the DNA modification by diol epoxide. Kinetic analysis implicates the formation of unstable phosphotriesters, hydrolysis of which nick the DNA.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Phosphotriester adducts (PTEs): DNA's overlooked lesion.
G. D. D. Jones, R. C. Le Pla, and P. B. Farmer (2010)
Mutagenesis 25, 3-16
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Detection of phosphodiester adducts formed by the reaction of benzo[a]pyrene diol epoxide with 2'-deoxynucleotides using collision-induced dissociation electrospray ionization tandem mass spectrometry.
M. Gaskell, B. Kaur, P. B. Farmer, and R. Singh (2007)
Nucleic Acids Res. 35, 5014-5027
   Abstract »    Full Text »    PDF »
Preferred sites of strand scission in DNA modified by andi-diol epoxide of benzo[a]pyrene.
W. Haseltine, K. Lo, and A. D'Andrea (1980)
Science 209, 929-931
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