Science, Vol 197, Issue 4298, 72-74
Copyright © 1977 by American Association for the Advancement of Science
Freeze-fractured purple membrane particles: protein content
KA Fisher
and
W Stoeckenius
Optical diffraction and image reconstruction can be used to correlate the electron microscope image of the biological membrane with its electron density projection. Such correlation shows that a single purple membrane particle contains 9 to 12 protein molecules--63 to 84 transmembrane alpha helices--a complexity two to ten times greater than that previously suggested for membrane particles.