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Science 24 June 1977:
Vol. 196. no. 4297, pp. 1439 - 1441
DOI: 10.1126/science.867038
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Articles

Science, Vol 196, Issue 4297, 1439-1441
Copyright © 1977 by American Association for the Advancement of Science

articles

Laser fluorimetry: subpicogram detection of aflatoxins using high-pressure liquid chromatography

GJ Diebold and RN Zare

The use of high-pressure liquid chromatographic separation in conjunction with laser-induced fluorescence detection permits the analysis of trace fluorescent species at new limits of sensitivity. This technique was applied to the carcinogens aflatoxins B1, B2, G1, and G2, which were linearly quantitated to 7.5 x 10(-13) gram. The procedure consists of forming more fluorescent aflatoxin derivatives, eluting the aflatoxins from a reverse-phase column, focusing the 325-nanometer output of a helium-cadmium ion laser into a suspended droplet of the eluent, and measuring the resulting fluorescence using phase-sensitive detection.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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R. Zare (1984)
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Attogram detection limit for aqueous dye samples by laser-induced fluorescence.
N. Dovichi, J. Martin, J. Jett, and R. Keller (1983)
Science 219, 845-847
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Liquid Chromatography in 1982.
D. H. Freeman and D. H. Freeman (1982)
Science 218, 235-241
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