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Science 10 June 1977:
Vol. 196. no. 4295, pp. 1221 - 1222
DOI: 10.1126/science.16344
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Articles

Science, Vol 196, Issue 4295, 1221-1222
Copyright © 1977 by American Association for the Advancement of Science

articles

Prelytic damage of red cells in filtrates from peroxidizing microsomes

MK Roders, EA Glende Jr, and RO Recknagel

When liver microsomes are incubated in the presence of reduced nicotinamide adenine dinucleotide phosphate (NADPH), their constituent lipids undergo peroxidative degeneration. If erythrocytes are present in such a peroxidizing system, they hemolyze. Filtrates obtained by ultrafiltration of peroxidizing microsomal systems were found to have the capacity to produce prelytic damage in red cells. Filtrates obtained from microsomes that had not undergone peroxidative lipid decomposition were inert. The toxic activity in the active filtrates was not due to continuing oxidation of NADPH nor to continuing liver microsomal lipid peroxidation. Neither the chemical identity of the toxic product or products in active filtrates nor the mechanisms involved in the erythrocyte damage are known at this time.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
trans-4-Hydroxy-2-hexenal: a reactive metabolite from the macrocyclic pyrrolizidine alkaloid senecionine.
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