Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 8 April 1977:
Vol. 196. no. 4286, pp. 197 - 200
DOI: 10.1126/science.847467
Prev | Table of Contents | Next

Articles

Science, Vol 196, Issue 4286, 197-200
Copyright © 1977 by American Association for the Advancement of Science

articles

Clones of individual repetitive sequences from sea urchin DNA constructed with synthetic Eco RI sites

RH Scheller, TL Thomas, AS Lee, WH Klein, WD Niles, RJ Britten, and EH Davidson

Interspersed repetitive sequences were isolated from sea urchin DNA by renaturing to low Cot followed by treatment with nuclease SI. Synthetic Eco RI sites were ligated onto the repetitive sequence elements, which were then inserted at the Eco RI site of plasmid RSF2124 and cloned. The repetitive sequences can be excised from the plasmid with Eco RI for further study.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Chemical DNA synthesis and recombinant DNA studies.
K Itakura and A. Riggs (1980)
Science 209, 1401-1405
   Abstract »    PDF »
Evolutionary conservation of repetitive sequence expression in sea urchin egg RNA's.
G. Moore, F. Costantini, J. Posakony, E. Davidson, and R. Britten (1980)
Science 208, 1046-1048
   Abstract »    PDF »
Regulation of gene expression: possible role of repetitive sequences.
E. Davidson and R. Britten (1979)
Science 204, 1052-1059
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)