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Science 8 April 1977:
Vol. 196. no. 4286, pp. 195 - 197
DOI: 10.1126/science.557836
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Articles

Science, Vol 196, Issue 4286, 195-197
Copyright © 1977 by American Association for the Advancement of Science

articles

Analysis of chicken ribosomal RNA genes and construction of lambda hybrids containing gene fragments

W McClements and AM Skalka

The ribosomal RNA genes (ribosomal DNA) of chicken are present in approximately 200 copies and are cleaved into two fragments of molecular weight 5 X 10(6) and 12 to 14 X 10(6) by restriction endonuclease Eco RI. Recombinant phages have been constructed in vitro by joining the smaller fragment of ribosomal DNA and the outer arms of DNA from the vector lambdagtWES-lambdaC. In one of the recombinants, the coding strand of the cloned fragment is in the proper orientation for transcription with lambda "early" genes; in the other two the orientation is reversed, with the coding strand in the proper position for transcription with lambda "late" genes.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Progress from Chicken Genetics to the Chicken Genome.
P. B. Siegel, J. B. Dodgson, and L. Andersson (2006)
Poult. Sci. 85, 2050-2060
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Avian Endogenous Proviral DNA: Analysis of Integrated ev 1 and a Related gs-chf- Provirus Purified by Molecular Cloning.
A. Skalka, P. DeBona, F. Hishinuma, and W. McClements (1980)
Cold Spring Harb Symp Quant Biol 44, 1097-1104
   Abstract »    PDF »
EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: the lambdagtWES system.
P Leder, D Tiemeier, and L Enquist (1977)
Science 196, 175-177
   Abstract »    PDF »


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