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Science 25 March 1977:
Vol. 195. no. 4284, pp. 1345 - 1348
DOI: 10.1126/science.841332
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Articles

Science, Vol 195, Issue 4284, 1345-1348
Copyright © 1977 by American Association for the Advancement of Science

articles

Enzyme polymorphisms as genetic signatures in human cell cultures

SU O'Brien, G Kleiner, R Olson, and JE Shannon

The electrophoretic resolution of seven relatively polymorphic human gene-enzyme systems expressed in tissue culture cells can be used as a sensitive genetic monitor for intraspecific cell contamination. An identical genotype at each of the same allozyme loci provides a 95% (or greater) confindence estimate of the identity of two cultured lines, on the basis of the allelic frequencies of the seven enzyme loci in natural populations and in populations of independently derived cultured cells. Of 27 commonly used human cell lines examined, only one of 351 pairwise comparisons proved genetically indistinguishable.


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Cross-contamination of cells in culture.
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T-1 cells are HeLa and not of normal human kidney origin.
W. Nelson-Rees, R. Flandermeyer, and D. Daniels (1980)
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Inter- and intraspecies contamination of human breast tumor cell lines HBC and BrCa5 and other cell cultures.
W. Nelson-Rees and R. Flandermeyer (1977)
Science 195, 1343-1344
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