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Science 4 March 1977:
Vol. 195. no. 4281, p. 891
DOI: 10.1126/science.190680
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Articles

Science, Vol 195, Issue 4281, 891-891
Copyright © 1977 by American Association for the Advancement of Science

articles

Superhelix densities of circular DNA's: a generalized equation for their determination by the bouyant method

WB Upholt

Equations for the measurement of the supertwisting of circular DNA's from banding positions in buoyant density gradients containing intercalating dyes have required the use of SV40 DNA isolated from virions as a reference DNA. These equations are modified to allow the use of any closed circular DNA of known superhelix density as a reference DNA.




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Science. ISSN 0036-8075 (print), 1095-9203 (online)