Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 4 February 1977:
Vol. 195. no. 4277, pp. 505 - 506
DOI: 10.1126/science.835013
Prev | Table of Contents | Next

Articles

Science, Vol 195, Issue 4277, 505-506
Copyright © 1977 by American Association for the Advancement of Science

articles

Ornithine decarboxylase may function as an initiation factor for RNA polymerase I

C Manen and DH Russel

Reparts suggest that the activity of RNA polymerase I is modulated by a labile protein with a hlaf-life of 10 to 20 minutes. Ornithine decarboxylase is the only labile protein (half-life, 10 to 20 minutes) that increases in activity prior to increased RNA polymerase I activity. The addition of a small amount of a highly purified ornithine decarboxylase preparation to an RNA polymerase I assay increases the initial rate of the reaction as well as the time for which the assay is linear. The incorporation patterns of 14C-labeled adenosine triphosphate and 32P-labeled adenosine triphosphate into RNA indicate that the addition of ornithine decarboxylase to the RNA polymerase assay increases the rate of initiation. This report demonstrates a novel way to purify ornithine decarboxylase by RNA polymerase I affinity chromatography and presents data in support of the hypothesis that the labile protein which modulates RNA polymerase I activity is ornithine decarboxylase.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Distribution Patterns of Ornithine Decarboxylase in Cells and Tissues: Facts, Problems, and Postulates.
R. G. Schipper and A. A.J. Verhofstad (2002)
J. Histochem. Cytochem. 50, 1143-1160
   Abstract »    Full Text »    PDF »
The Nucleolus, a Model for Analysis of Chromatin Controls.
H. Busch, N. R. Ballal, R. K. Busch, Y. C. Choi, F. Davis, I. L. Goldknopf, S.-I. Matsui, M. S. Rao, and L. I. Rothblum (1978)
Cold Spring Harb Symp Quant Biol 42, 665-683
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)