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Science 4 February 1977:
Vol. 195. no. 4277, pp. 494 - 495
DOI: 10.1126/science.835009
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Articles

Science, Vol 195, Issue 4277, 494-495
Copyright © 1977 by American Association for the Advancement of Science

articles

Antibody binding measurements with hapten-selective membrane electrodes

M Meyerhoff and GA Rechnitz

Direct antibody-hapten binding measurements were carried out with the use of a hapten-selective membrane electrode. Potentiometric titrations of rabbit antibody to the hapten trimethylphenylammonium ion demonstrate the speed and convenience of the new method for the determination of binding equilibria. Average intrinsic binding constants obtained agree well with those measured by equilibrium dialysis techniques.




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Science. ISSN 0036-8075 (print), 1095-9203 (online)