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Science 28 January 1977:
Vol. 195. no. 4276, pp. 391 - 393
DOI: 10.1126/science.318763
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Articles

Science, Vol 195, Issue 4276, 391-393
Copyright © 1977 by American Association for the Advancement of Science

articles

Novel screening procedure for recombinant plasmids

J Telford, P Boseley, W Schaffner, and M Birnstiel

Lysed bacterial colonies containing potential recombinant plasmids were mixed with molten agar and sealed into slots of an agarose gel. After electrophoresis overnight, the gel was stained with ethidium bromide, which clearly reveals recombinant plasmids. Xenopus laevis ribosomal DNA and histone DNA of Psammechinus miliaris were ligated into pCRI plasmids and screened by this method.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Deletion Mutants of SV40 That Affect the Structure of Viral Tumor Antigens.
J. M. Pipas, S. P. Adler, K. W. C. Peden, and D. Nathans (1980)
Cold Spring Harb Symp Quant Biol 44, 285-291
   Abstract »    PDF »
Plasmid detection and sizing in single colony lysates.
W. Barnes (1977)
Science 195, 393-394
   Abstract »    PDF »


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