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Science 21 January 1977:
Vol. 195. no. 4275, pp. 289 - 292
DOI: 10.1126/science.63993
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Articles

Science, Vol 195, Issue 4275, 289-292
Copyright © 1977 by American Association for the Advancement of Science

articles

Oncornavirus: isolation from a squirrel monkey (Saimiri sciureus) lung culture

RL Heberling, ST Barker, SS Kalter, GC Smith, and RJ Helmke

An oncornavirus isolated from a squirrel monkey (Saimiri sciureus) lung culture has a density of 1.16 to 1.17 grams per milliliter, contains 70S RNA, and has an RNA-directed DNA polymerase that prefers Mg2+ over Mn2+ in an assay in which polyribocytidylate - oligodeoxyguanylate (12-18) is used as a synthetic template. Morphologically, the virus resembles Mason-Pfizer monkey virus but is antigenically distinct from this virus. The virus grows in cells of human, chimpanzee, rhesus monkey, canine, and mink origin, but not cells of squirrel monkey origin. On the basis of its properties, the newly isolated virus can be classified as a retravirus.


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