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Science 19 November 1976:
Vol. 194. no. 4267, pp. 848 - 851
DOI: 10.1126/science.790569
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Articles

Science, Vol 194, Issue 4267, 848-851
Copyright © 1976 by American Association for the Advancement of Science

articles

A specific population of gonadotrophs purified from immature female rat pituitary

C Denef, E Hautekeete, and L Rubin

When dispersed pituitary cells from 14-day-old female rats were sedimented in a bovine serum albumin gradient, a fraction was isolated which consisted of almost 90 percent of large cells that stained purple in the periodic acid-Schiff (PAS) reaction. Immunostaining indicated that over 85 percent of these PAS-purple cells were gonadotrophs containing both follicle stimulating and luteinizing hormone. Reproducible cell cultures could be obtained on poly-L-lysine coated dishes. As early as the second day in culture, the secretion of both follicle stimulating and luteinizing hormone was highly stimulated by luteinizing hormone-releasing hormone. The effect of FSH was as marked as that on LH. The data suggest that the isolated gonadotrophs are a specific functional subtype.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
{beta}1-Adrenoceptor Expression in Rat Anterior Pituitary Gonadotrophs and in Mouse {alpha}T3-1 and L{beta}T2 Gonadotrophic Cell Lines.
K. Janssens, O. Krylyshkina, N. Hersmus, H. Vankelecom, and C. Denef (2008)
Endocrinology 149, 2313-2324
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Complete Sertoli Cell Proliferation Induced by Follicle-Stimulating Hormone (FSH) Independently of Luteinizing Hormone Activity: Evidence from Genetic Models of Isolated FSH Action.
C. M. Allan, A. Garcia, J. Spaliviero, F.-P. Zhang, M. Jimenez, I. Huhtaniemi, and D. J. Handelsman (2004)
Endocrinology 145, 1587-1593
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Developmental Changes in the Hormonal Identity of Gonadotroph Cells in the Rhesus Monkey Pituitary Gland.
D. Meeran, H. F. Urbanski, S. J. Gregory, J. Townsend, and D. J. Tortonese (2003)
J. Clin. Endocrinol. Metab. 88, 2934-2942
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Characterization and Distribution of Gonadotrophs in the Pars Distalis and Pars Tuberalis of the Equine Pituitary Gland During the Estrous Cycle and Seasonal Anestrus.
R. C. Eagle and D. J. Tortonese (2000)
Biol Reprod 63, 826-832
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