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Science 12 November 1976:
Vol. 194. no. 4266, pp. 743 - 745
DOI: 10.1126/science.982044
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Articles

Science, Vol 194, Issue 4266, 743-745
Copyright © 1976 by American Association for the Advancement of Science

articles

Plasma membrane vesiculation: a new technique for isolation of plasma membranes

RE Scott

Monolayer cell cultures of macrophages, monocytes, myoblasts, and density-inhibited and transformed fibroblasts form and release cell surface membrane vesicles following exposure to formaldehyde, related low-molecular-weight aldehydes, and disulfide blocking agents. Vesicles have a unique composition of proteins and lipids. They show enrichment of cholesterol and sphingomyelin content and a seven-to tenfold enrichment of 5'-nucleotidase activity. Vesicles also contain intramembranous particles and show a trilamellar unit membrane and no ultrastructural evidence of contamination with other cytoplasmic organelles. The technique is proposed as a novel method for isolating plasma membrane vesicles from cells in culture.


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