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Science 30 July 1976:
Vol. 193. no. 4251, pp. 401 - 403
DOI: 10.1126/science.935875
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Articles

Science, Vol 193, Issue 4251, 401-403
Copyright © 1976 by American Association for the Advancement of Science

articles

Interkingdom fusion between human (HeLa) cells and tobacco hybrid (GGLL) protoplasts

CW Jones, IA Mastrangelo, HH Smith, HZ Liu, and RA Meck

The fusion of human HeLa cells with tobacco protoplasts has been accomplished with the use of polyethylene glycol. The sequence from heterocellular adherence to heterokaryon formation has been followed with light microscopy and confirmed by autoradiographs of heterokaryons containing unlabeled tobacco nuclei and tritium-labeled HeLa nuclei. The HeLa nucleus retained its integrity in the tobacco cytoplasm up to 6 days after fusion.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Manipulating the genetic identity and biochemical surface properties of individual cells with electric-field-induced fusion.
A. Stromberg, F. Ryttsen, D. T. Chiu, M. Davidson, P. S. Eriksson, C. F. Wilson, O. Orwar, and R. N. Zare (2000)
PNAS 97, 7-11
   Abstract »    Full Text »    PDF »
Plant Genetics: Increasing Crop Yield.
P. R. Day (1977)
Science 197, 1334-1339
   Abstract »    PDF »


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