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Science 4 June 1976:
Vol. 192. no. 4243, pp. 1014 - 1016
DOI: 10.1126/science.775637
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Articles

Science, Vol 192, Issue 4243, 1014-1016
Copyright © 1976 by American Association for the Advancement of Science

articles

Human pancreatic islets in culture: effects of supplementing the medium with homologous and heterologous serum

H Goldman and E Colle

Islets of Langerhans, isolated from the human fetal pancreas and cultured in media supplemented with serums from human umbilical cord or adult donors, remained intact, free-floating, and functional for 2 to 5 months. Substitution of fetal calf serum for the human serum usually resulted in attachment of islets, monolayer formation, and relatively early (3 weeks) functional arrest.




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Science. ISSN 0036-8075 (print), 1095-9203 (online)