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Science 21 May 1976:
Vol. 192. no. 4241, pp. 793 - 794
DOI: 10.1126/science.1265480
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Articles

Science, Vol 192, Issue 4241, 793-794
Copyright © 1976 by American Association for the Advancement of Science

articles

Uptake of bovine serum albumin by rainbow trout from hypersmotic solutions: a model for vaccinating fish

DF Amend and DC Fender

Immersion of juvenile rainbow trout (Salmo gairdneri) in a solution containing either urea or sodium chloride at 1650 milliosmoles and 2 percent of bovine serum albumin (BSA) resulted in an uptake of BSA into the blood of the fish after a 3-minute exposure. Similar blood levels of BSA were also obtained by placing the fish in 1650 millosmoles of sodium chloride for about 2 minutes, and then immersing them in 2 percent BSA solution for 3 minutes. Uptake of BSA into the fish appeared to be primarily through the lateral line system and secondarily through the gills.




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Science. ISSN 0036-8075 (print), 1095-9203 (online)