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Science 30 April 1976:
Vol. 192. no. 4238, pp. 487 - 489
DOI: 10.1126/science.1257785

Articles

Science, Vol 192, Issue 4238, 487-489
Copyright © 1976 by American Association for the Advancement of Science


articles

Lens culinaris lectin immobilized on sepharose: binding and sugar-specific release of intact tissue culture cells

V Kinzel, D Kubler, J Richards, and M Stohr

Lens culinaris lectin (LCL) covalently linked to 2B Sepharose binds tissue culture cells to the matrix. This is prevented by hapten sugars specific for LCL. Unlike other immobilized lectins, lens culinaris lectin allows the removal of bound cells from the matrix on addition of the specific sugars in a concentration-dependent manner. Binding and release occur under physiological conditions. Released cells continue to grow.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Expression of the Functional Extracellular Domain of Human Thyrotropin Receptor Using a Vaccinia Virus System: Its Purification and Analysis of Autoantibody Binding.
M. H. Lee, J. Y. Park, B. Y. Cho, and C.-B. Chae (1999)
J. Clin. Endocrinol. Metab. 84, 1391-1397
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