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Science 26 March 1976:
Vol. 191. no. 4233, pp. 1266 - 1268
DOI: 10.1126/science.1083070
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Articles

Science, Vol 191, Issue 4233, 1266-1268
Copyright © 1976 by American Association for the Advancement of Science

articles

A cytoplasmic factor promoting oocyte maturation: its extraction and preliminary characterization

WJ Wasserman and Y Masui

Cytoplasm of maturing amphibian oocytes possesses a factor that induces germinal vesicle breakdown. This factor was extracted from Rana pipiens eggs and assayed by microinjection in Xenopus laevis oocytes. The activity of this factor is Mg-dependent, Ca-sensitive, and associated with heat-labile protein. Centrifugation on a sucrose density gradient revealed that the factor exists in three different molecular sizes.


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