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Science 19 March 1976:
Vol. 191. no. 4232, pp. 1178 - 1179
DOI: 10.1126/science.191.4232.1178
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Articles

Shoot Initiation from Carnation Shoot Apices Frozen to –196°C

MICHAEL SEIBERT 1

1 GTE Laboratories, Inc., Waltham, Massachusetts 02154

Aseptically excised shoot apices from Dianthus caryophyllus were frozen to –196°C and subsequently thawed to room temperature. Survival rates as high as 33 percent, determined by callus formation, growth, or chlorophyll formation, are reported; plant formation has been observed.

Submitted on October 20, 1975
Revised on December 2, 1975

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Science. ISSN 0036-8075 (print), 1095-9203 (online)