Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 6 February 1976:
Vol. 191. no. 4226, pp. 466 - 468
DOI: 10.1126/science.1246629
Prev | Table of Contents | Next

Articles

Science, Vol 191, Issue 4226, 466-468
Copyright © 1976 by American Association for the Advancement of Science

articles

Measurement of membrane protein lateral diffusion in single cells

M Edidin, Y Zagyansky, and TJ Lardner

Fluorescence rapidly returns to spots bleached by a laser beam in the continuous fluorescence of cultured cells labeled on the surface with fluorescein isothiocyanate. The rate of recovery of fluorescence after bleaching can be interpreted as a measure of the lateral diffusion of integral membrane proteins labeled with fluorescein.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The anchoring protein SAP97 retains Kv1.5 channels in the plasma membrane of cardiac myocytes.
J. Abi-Char, S. El-Haou, E. Balse, N. Neyroud, R. Vranckx, A. Coulombe, and S. N. Hatem (2008)
Am J Physiol Heart Circ Physiol 294, H1851-H1861
   Abstract »    Full Text »    PDF »
The effect of cellular cholesterol on membrane-cytoskeleton adhesion.
M. Sun, N. Northup, F. Marga, T. Huber, F. J. Byfield, I. Levitan, and G. Forgacs (2007)
J. Cell Sci. 120, 2223-2231
   Abstract »    Full Text »    PDF »
Aqueous humor outflow: what do we know? Where will it lead us?.
M. P. Fautsch, D. H. Johnson, and the Second ARVO/Pfizer Research Institute Working (2006)
Invest. Ophthalmol. Vis. Sci. 47, 4181-4187
   Full Text »    PDF »
Kinetic and Molecular Analysis of Nuclear Export Factor CRM1 Association with Its Cargo In Vivo.
D. Daelemans, S. V. Costes, S. Lockett, and G. N. Pavlakis (2005)
Mol. Cell. Biol. 25, 728-739
   Abstract »    Full Text »    PDF »
Function recovery after chemobleaching (FRAC): Evidence for activity silent membrane receptors on cell surface.
H. Sun, S. Shikano, Q. Xiong, and M. Li (2004)
PNAS 101, 16964-16969
   Abstract »    Full Text »    PDF »
In Vivo HIV-1 Rev Multimerization in the Nucleolus and Cytoplasm Identified by Fluorescence Resonance Energy Transfer.
D. Daelemans, S. V. Costes, E. H. Cho, R. A. Erwin-Cohen, S. Lockett, and G. N. Pavlakis (2004)
J. Biol. Chem. 279, 50167-50175
   Abstract »    Full Text »    PDF »
Intracellular Macromolecular Mobility Measured by Fluorescence Recovery after Photobleaching with Confocal Laser Scanning Microscopes.
J. Braga, J. M.P. Desterro, and M. Carmo-Fonseca (2004)
Mol. Biol. Cell 15, 4749-4760
   Abstract »    Full Text »    PDF »
Lipids in the inner membrane of dormant spores of Bacillus species are largely immobile.
A. E. Cowan, E. M. Olivastro, D. E. Koppel, C. A. Loshon, B. Setlow, and P. Setlow (2004)
PNAS 101, 7733-7738
   Abstract »    Full Text »    PDF »
Computational imaging in cell biology.
R. Eils and C. Athale (2003)
J. Cell Biol. 161, 477-481
   Abstract »    Full Text »    PDF »
A soluble protein is immobile in dormant spores of Bacillus subtilis but is mobile in germinated spores: Implications for spore dormancy.
A. E. Cowan, D. E. Koppel, B. Setlow, and P. Setlow (2003)
PNAS 100, 4209-4214
   Abstract »    Full Text »    PDF »
Nuclear Transport of Parathyroid Hormone (PTH)-Related Protein Is Dependent on Microtubules.
M. H. C. Lam, R. J. Thomas, K. L. Loveland, S. Schilders, M. Gu, T. J. Martin, M. T. Gillespie, and D. A. Jans (2002)
Mol. Endocrinol. 16, 390-401
   Abstract »    Full Text »    PDF »
TCR dynamics on the surface of living T cells.
B. Favier, N. J. Burroughs, L. Wedderburn, and S. Valitutti (2001)
Int. Immunol. 13, 1525-1532
   Abstract »    Full Text »    PDF »
Constraint of the translational diffusion of a membrane glycoprotein by its external domains.
M Wier and M Edidin (1988)
Science 242, 412-414
   Abstract »    PDF »
Journey to the center of the cell: role of the receptosome.
I. Pastan and M. Willingham (1981)
Science 214, 504-509
   Abstract »    PDF »
Lateral diffusion of surface molecules in animal cells and tissues.
W. Gall and G. Edelman (1981)
Science 213, 903-905
   Abstract »    PDF »
Denervated skeletal muscle fibers develop discrete patches of high acetylcholine receptor density.
P. Ko, M. Anderson, and M. Cohen (1977)
Science 196, 540-542
   Abstract »    PDF »
Lateral diffusion in planar lipid bilayers.
P. Fahey, D. Koppel, L. Barak, D. Wolf, E. Elson, and W. Webb (1977)
Science 195, 305-306
   Abstract »    PDF »
Lateral transport of a lipid probe and labeled proteins on a cell membrane.
J Schlessinger, D Axelrod, D. Koppel, W. Webb, and E. Elson (1977)
Science 195, 307-309
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)