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Science 17 October 1975:
Vol. 190. no. 4211, pp. 282 - 284
DOI: 10.1126/science.1179208
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Articles

Science, Vol 190, Issue 4211, 282-284
Copyright © 1975 by American Association for the Advancement of Science

articles

Human interferon production: superinduction by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole

PB Sehgal, I Tamm, and J Vilcek

Polyinosinic.polycytidylic acid [poly(I.C)] induced production of interferon by a strain of diploid human fibroblasts (FS-4), measured between 5 and 24 hours from induction, is enhanced up to 128-fold by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB), a reversible inhibitor of nuclear heterogeneous RNA synthesis. A normalized dose-effect plot shows a close correlation between the superinducing effect of DRB and inhibition of RNA synthesis. Cultures that contained DRB continue to produce interferon for up to 4 days. Removal of the drug at any time during this period leads to a prompt shutoff of interferon production.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
5,6-Dichloro-1-Beta-D-ribofuranosylbenzimidazole inhibits initiation of nuclear heterogeneous RNA chains in HeLa cells.
P. Sehgal, E Derman, G. Molloy, I Tamm, and J. Darnell (1976)
Science 194, 431-433
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